Tissue repair
TB-500 research overview
TB-500 is the synthetic version of the Thymosin Beta-4 fragment, studied for its role in actin regulation, cellular migration and tissue recovery mechanisms.
5 min read
What is TB-500?
TB-500 refers to the synthetic form of the active fragment of Thymosin Beta-4 (Tβ4), a 43-amino-acid actin-sequestering protein. The synthetic fragment corresponds to amino acids 17–23 of Tβ4 (Ac-LKKTETQ). CAS: 885340-08-9.
Thymosin Beta-4 is produced by platelets, white blood cells and other cell types and is released in high concentrations following tissue injury. Research has focused on its role as a promoter of angiogenesis, cellular migration, and inflammatory regulation.
Mechanism of action
TB-500 functions primarily through its interaction with G-actin (globular actin). By binding monomeric actin and preventing its polymerisation into F-actin filaments, Tβ4 regulates cytoskeletal dynamics critical to cell migration.
In tissue-repair models, this promotes keratinocyte and endothelial cell migration into the repair site. TB-500 also appears to upregulate matrix metalloproteinases (MMPs) and modulate inflammatory mediators, facilitating extracellular matrix remodelling.
Animal model research
Published rodent and large animal studies have examined TB-500 in:
- Cardiac muscle repair following myocardial infarction (mouse/rat)
- Dermal repair (full-thickness excision models)
- Corneal repair
- Traumatic brain injury (mouse)
- Tendon injury (horse — naturally occurring Tβ4)
As with other research peptides, translation to human clinical outcomes has not been established and no human clinical data exists.
Reconstitution and storage
Store lyophilized TB-500 at −20 °C. Reconstitute with bacteriostatic water immediately before use. Reconstituted solutions are stable for 28 days at 4 °C. Avoid light exposure and repeated freeze-thaw.
Research this compound
TB-500
99.63% purity · RP-HPLC